Extended Data Fig. 4: Bone marrow angiogenesis in response to myocardial infarction.

a, Experimental outline. b, Image of the heart’s infarct border zone showing the formation of new ZsGreen⁺ blood vessels after MI (n=3 animals). c, Serial intravital immunofluorescence imaging of Tamoxifen-treated Apln^CreER;Rosa26^ZsGreen/+ mice 1 day before and 6 days after MI, showing newly formed ZsGreen-labelled blood vessels (arrows; n=3 animals per group). d, Images of the femur 6 days after MI, showing CD31⁺Sca-1⁺ vasculature, newly formed ZsGreen⁺ blood vessels and CD11b⁺ myeloid cells (n=5 animals). e, Distances from leukocyte-containing CD11b⁺ pixels to CD31⁺Sca-1⁺ and to newly formed ZsGreen⁺ vasculature in femora of control and mice after MI (n=17 regions of n=5 animals 6 days post MI, multiple two-tailed t-tests). f, In vivo calvarial bone marrow imaging after transplantation of DiD-labelled hematopoietic progenitor cells (LSK, Lin⁻ Sca-1⁺ c-kit⁺) in Tamoxifen-treated Apln^CreER;Rosa26^ZsGreen/+ mice (naive controls and day 6 after MI). Circles indicate individual DiD-labelled cells (n=3 animals per group). g, Mean target-to-background ratios (TBR) of individual DiD-labelled cells at a distance of either <40µm or >40µm to the closest ZsGreen-labelled new blood vessel in controls and mice after MI (control <40µm: n=92 cells analyzed; control >40µm: n=163; MI <40µm: n=98; MI >40µm: n=90; n=3 mice per group; two-way ANOVA with Sidak’s post-test). Data are displayed as mean±SEM.