Extended Data Fig. 7: Gene deletion in bone marrow endothelial cells.

a, Relative Il6 mRNA levels assessed by qPCR in bone marrow endothelial cells (BMEC) FACS-isolated from mice with saline or Angiotensin II (HTN) for 8 weeks (n=5 mice per group), from wild type (Control) and Apoe^−/− mice on a Western Diet for 10 weeks (Athero, n=6 mice per group), and from controls and mice on day 4 after MI (n=6 mice per group, two-tailed Mann-Whitney test). b, c, Concentration of Il6 in blood plasma (b) and bone marrow interstitial fluid (c) in wild type (Control) and Apoe^−/− mice on a Western Diet for 10 weeks (Athero) by ELISA (n=5 controls, n=7 Apoe^−/−, two-tailed Mann-Whitney test). d, Experimental outline. e, Flow cytometry enumeration of splenic LSK and myeloid cells in Cdh5^CreERT2 and Cdh5^CreERT2;Il6^fl/fl mice 12 weeks after PCSK9-AAV injection and start of an atherogenic diet in order to induce atherosclerosis (n=6 Cdh5^CreERT2, n=5 Cdh5^CreERT2;Il6^fl/fl mice, two-tailed Mann-Whitney test). f, Vascular permeability measured by Evans Blue extravasation in the femoral bone marrow of Cdh5^CreERT2 and Cdh5^CreERT2;Il6^fl/fl mice with atherosclerosis (n=6 per group, two-tailed Mann-Whitney test). g, Versican (Vcan) expression in flow-sorted bone marrow endothelial cells from mice with saline or Angiotensin II (HTN) for 8 weeks (n=5 mice per group), from wild type (Control) and Apoe^−/− mice on a Western Diet for 10 weeks (n=6 mice per group) and from controls and mice 4 days after MI (n=6 mice per group, two-tailed Mann-Whitney tests). h, Experimental outline. i, Flow cytometry enumeration of splenic LSK and myeloid cells in Cdh5^CreERT2 and Cdh5^CreERT2;Vcan^fl/fl mice 3 days after MI (n=4 mice per group, two-tailed Mann-Whitney test). j, Vascular permeability measured by Evans Blue extravasation in the femoral bone marrow of Cdh5^CreERT2 and Cdh5^CreERT2;Vcan^fl/fl mice on day 2 after MI (n=5 mice per group, two-tailed Mann-Whitney test). Data are displayed as mean±SEM.