Extended Data Fig. 5: Cardiomyocyte sub-cluster 0 specific signatures.
From: A human cell atlas of the pressure-induced hypertrophic heart
(a) Cardiomyocyte representation in cluster CM0 as percentage of all cardiomyocytes shown for each individual patient (Healthy n=14; AS n=5). (b) Violin plots for the genes ERBB4, TBX20 and GATA4 in cardiomyocytes of cluster 0 only (log2 transformed and normalized UMI counts). (c) Violin plots for the genes ADRA1A, NLGN1 and FGF12 in cardiomyocytes of cluster 0 only (log2 transformed and normalized UMI counts). Data are shown as mean ± s.e.m. (a). Normal distribution was assessed using the Kolmogorov-Smirnov test (a). Statistical analysis to compare two Gaussian distributed groups was performed using the unpaired, two-sided Student’s t-test (a). t=3,013, 17 degrees of freedom (a). Adjusted p-values based on Bonferroni correction using all genes of the dataset to compare the expression in violin plots were calculated with the Seurat function ‘FindAllMarkers’ using ‘bimod’ as the statistical test (b-c).
