Extended Data Fig. 6: Relative expression profiles of FHF1-4 isoforms/splice-variants in iPSC-CMs. | Nature Cardiovascular Research

Extended Data Fig. 6: Relative expression profiles of FHF1-4 isoforms/splice-variants in iPSC-CMs.

From: Fibroblast growth factor homologous factors serve as a molecular rheostat in tuning arrhythmogenic cardiac late sodium current

Extended Data Fig. 6

a, Analysis of relative expression of FHF1-4 isoforms/splice-variants using RT-qPCR demonstrate FHF1A, FHF1B, and FHF2S as the major isoforms in healthy-donor iPSC-CMs. We observed that FHF2S expression was ~ 3-fold higher than FHF1B in the healthy-donor iPSC-CMs while FHF1A was ~ 45% lower than FHF1B. Each bar and error, mean ± SEM. n = 3 independent cultures. (see Supplementary Table 1). b, In the LQTS3 (ΔKPQ) iPSC-CMs, FHF1B is the major isoform/splice-variant with ~ 50% lower level of FHF1A and ~ 20% lower level of FHF2S. The relative prevalence of FHF1B in the LQTS3 (ΔKPQ) iPSC-CMs is consistent with the high baseline INa,L obtained in functional studies in Fig. 5. Each bar and error, mean ± SEM. n = 5 independent cultures. (see Supplementary Table 1).

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