Fig. 5: Calcineurin-NFAT signaling pathway in αMHC-MCM+/− and Piezo1 KO mouse hearts 2 d post-TAC.

a, Representative western blots showing the expression of NFAT and GATA4 in cytoplasm (left) and nucleus (right). b, Cytoplasmic and nuclear (right) quantitative data were normalized by GAPDH and Histone H2B, respectively (n = 6 per group). Fold changes and nuclear/cytoplasmic ratio were calculated relative to sham groups, in each genotype. Results are presented as mean ± s.e.m.; Welch’s t-test, two-tailed, was used for comparisons between the two groups for b; **P < 0.01, ***P < 0.001 versus sham-operated group.