Fig. 6: Gene expression and protein levels of selected Ca2+ handling molecules 2 d after TAC in Piezo1 KO and αMHC-MCM+/− mouse hearts.
a–f, Relative mRNA expression of: Trpm4 (a), Piezo1 (b), SERCA2a (c), PLN (d) and Slc8a1 in Piezo1 KO (e) and Slc8a1 in Trpm4 KO (f) in LV tissue 2 d after sham or TAC. g, Representative western blots of TRPM4, SERCA2a, PLN, p-PLN T17 and NCX1 in LV tissue. h–o, Western blots from LV tissue after 2 d of TAC were used to quantify the protein levels of: TRPM4 (h), SERCA2a (i), PLN (j), p-PLN T17 (k), the ratio of SERCA2a/PLN (l), the ratio of p-PLN T17/PLN (m) and NCX1 in Piezo1 KO (n) and NCX1 in TRPM4 KO (o). Relative mRNA (n = 5 per group) and protein expression (n = 6 per group) in the LV tissue were normalized to GAPDH and calculated as fold change relative to the 2-d post-sham group, respectively. Results are presented as mean ± s.e.m.; two-way ANOVA with Tukey’s post hoc test for multiple comparisons was used to assess effects of genotype, surgery and genotype by surgery interaction for a–f; and Welch’s t-test, two-tailed, was used for h–o; *P < 0.05, **P < 0.01, ***P < 0.001 versus sham-operated groups for the respective genotypes; ###P < 0.001 versus TAC groups.
