Fig. 2: scRNA-seq of HUCMSCs reveals four MSC clusters and a transcriptome enhanced for regeneration, anti-inflammation, cell cycle and metabolism. | Nature Cardiovascular Research

Fig. 2: scRNA-seq of HUCMSCs reveals four MSC clusters and a transcriptome enhanced for regeneration, anti-inflammation, cell cycle and metabolism.

From: Human umbilical cord mesenchymal stem cell-derived treatment of severe pulmonary arterial hypertension

Fig. 2: scRNA-seq of HUCMSCs reveals four MSC clusters and a transcriptome enhanced for regeneration, anti-inflammation, cell cycle and metabolism.The alternative text for this image may have been generated using AI.

a, Uniform manifold approximation and projection (UMAP) graph of the first two UMAP dimensions shows four clusters of MSCs. Based on the pathway/GO annotation of their marker genes, the functional labels were added to the graph. MSC-like cells were isolated from the human umbilical cord (Wharton’s jelly) after delivery of a full-term infant (38-week gestation). After MSC culture, MSCs were harvested in passage 3 to undergo scRNA-seq, as described in the Methods. b, LC–MS chromatogram showing the mass spectrometric traces of PGE2, PGF2α and the internal standard d4-PGE2. c–f, EDTA plasma concentrations of NEDD9, ICAM-1, SAA and IFN-γ (mean of concentrations near-simultaneously measured in the SVC, IVC and RA). AU, arbitrary units.

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