Extended Data Fig. 3: CD155 expression in tissue macrophages and in monocyte-derived macrophages.
a. Single cell suspensions were generated from human arthrosclerotic arteries and analyzed by flow cytometry. Gating strategy to identify CD68 + macrophages. Corresponded to panel b, c, and Extended data Fig. 6. b. Representative plot graphs from 2 tissues defining co-expression of CD206 and HLA-DR. c. Surface expression of CD155 on different tissue macrophage clusters. Data from two tissues are shown. d. Monocyte-derived macrophages (Mϕ) were treated with either LPS (100 ng/ml)+IFN-g(100 u/ml) or IL-4 (20 ng/ml). Surface CD155 expression was determined by FACS. Representative histograms and mean ± SEM of mean fluorescence intensities from 6 experiments. Two-tailed student t test. P value shown on figure.
