Extended Data Fig. 4: Efficiency of CD155 knockdown and impact of CD155 on anti-viral immune responses in healthy individuals.
a. Monocyte-derived macrophages (Mϕ) were generated from patients and controls. Relative expression CD155 transcripts in healthy and CAD Mϕ (n = 12). Mϕ were generated as in Fig. 2 and transfected with control or CD155 siRNA. b. CD155 transcripts were quantified by qPCR. c. CD155 expression on the surface of Mϕ detected by flow cytometry. n = 8. T cells from healthy individuals were stimulated with viral antigen-loaded Mϕ. In parallel cultures, anti-CD155 antibodies were added or CD155 was knocked down by siRNA technology. Six hours later, antigen-responsive CD69+ CD40L+ T cells were measured by flow cytometry. d. IFN-γ secretion after SARS-CoV2 antigen stimulation in the absence and presence of anti-CD155 antibodies. n = 10 each. e. IFN-γ secretion after EBV antigen stimulation in the absence and presence of anti-CD155 antibodies. n = 5 each. f. Frequencies of anti-SARS-CoV2 CD69+ CD40L+ T cells after CD155 blockade. n = 8. g. Frequencies of anti-EBV CD69+ CD40L+ T cells after CD155 blockade. n = 5. h. IFN-γ release induced by SARS-CoV2 antigen-pulsed CD155 siRNA-transfected Mϕ. n = 10. i. IFN-γ release induced by EBV antigen-pulsed CD155 siRNA-transfected Mϕ. n = 5. j. Frequencies of anti-SARS-CoV2 CD69+ CD40L+ T cells after CD155 knockdown. n = 8. k. Frequencies of anti-SARS-CoV2 CD69+ CD40L+ T cells after CD155 knockdown. n = 5. Individual data points are displayed. Data are mean ± SEM. Differences were compared with 2 tail student t test. p-value was shown on each panel.
