Extended Data Fig. 5: Macrophage survival.
a.-d. Monocytes were isolated and differentiated into macrophages (Mϕ) with M-CSF over 5 days. Cell survival rates were quantified by 3 different approaches: a.-b. Simultaneous Live/dead cell staining with calcein AM (live) and BOBO-3 (dead). (a) Representative image from 3 independent experiments. (e) Cell survival rates from 12 controls and 12 patients. c. Cell death was measured by LDH release. Data from 12 controls and 12 patients. d. Cell viability was measured by staining with the alamarBlue cell viability reagent. Data from 12 controls and 12 patients. e.-h. Monocyte-derived macrophages (Mϕ) were transfected with control siRNA, CD155-specific si-RNA or METTL3-specific siRNA. Cell survival was quantified after 48 hrs. (e) Calcein AM (live) / BOBO-3 (dead) staining. Representative images from 8 experiments in each group. (f) Cell death rates measured in all 8 experiments. (g) LDH release measured in all 8 experiments. (h) AlamarBlue cell viability staining. Data from 8 experiments. Individual data points are displayed. Data are mean ± SEM. Differences were compared with one-way ANOVA.
