Extended Data Fig. 2: Collateral artery assessments. | Nature Cardiovascular Research

Extended Data Fig. 2: Collateral artery assessments.

From: Blood flow modeling reveals improved collateral artery performance during the regenerative period in mammalian hearts

Extended Data Fig. 2: Collateral artery assessments.

a,b, Maximum intensity projection of an entire neonate and adult heart 4-days post myocardial infarction (MI) immunolabeled with α-SMA. (a) P6 neonatal heart. (b) Adult (12-week-old) heart. c, Collateral numbers in neonate (n=9 hearts) and adult (n=6 hearts) 4 days post-MI. d–f, Evaluation of collateral patency in P6 neonatal heart 4-days post MI. (d) Schematic of staining method. (e) 3D reconstruction of α-SMA+ collaterals (purple) overlaid with α-SMA and CD31 staining. (f) MIPs of representative Z-stacks (30 μm) within e highlighting (solid purple arrow) a patent (α-SMA+ and CD31+) collateral. Scale bars: a and b 1 mm; e 500 μm. Error bars are mean ± st dev: ****, p≤0.0001 by two-sided Student’s t-test.

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