Extended Data Fig. 6: CREs within mesoderm cell subpopulations are associated with distinct transcription factor binding activity.
From: Human-gained heart enhancers are associated with species-specific cardiac attributes
a, Clustered heatmap shows differentially (FDR < 0.05) accessible CREs between identified snATAC mesoderm cell subpopulations. b, Heatmap of transcription factor binding motif analysis of cluster-specific CREs from human mesodermal snATAC-seq data reveals that ZIC3 along with other cardiac developmental transcription factor motifs are enriched in the pre-cardiac mesoderm subpopulation. c, ZIC3 motif enrichment is projected onto the UMAP of human PSC-derived mesoderm snATAC-seq data. d, (Left) Mouse scRNA-seq data from E7.25-E8.5 Mesp1-Cre; Rosa26-tdTomato embryos are displayed by tSNE (t-distributed stochastic neighbor embedding) plots for all developmental stages combined. (Right) Zic3 expression is projected onto the tSNE plot. PSC, pluripotent stem cell; N, neural; Epi, epiblast; ExE, extra-embryonic; APS/ME, anterior primitive streak/mesendoderm; LPM, lateral-plate mesoderm; PCM, pre-cardiac mesoderm; PM; paraxial mesoderm, PSM, pre-somitic mesoderm; CRE, cis-regulatory region; adj., adjusted.
