Extended Data Fig. 7: Generation of clonal ZIC3 HGE deletion lines.
From: Human-gained heart enhancers are associated with species-specific cardiac attributes
Human PSC ZIC3 HGE1-3 individual knockout (KO) and triple KO (TKO) lines were generated through CRISPR/Cas9-mediated deletion of ZIC3 HGE1-3 enhancer regions (9.2 kb, 1.8 kb, and 1.5 kb, respectively). PCR genotyping using external (ext., that is, outside deleted regions) and internal (int., that is, inside deleted regions) primers confirmed the individual human PSC ZIC3 HGE KOs (left) and the human PSC ZIC3 HGE TKO (right). HGE TKO was confirmed by individual HGE1-3 genotyping. Wild-type (WT) cells were used as control. Note that the WT HGE1 band is 9.2 kb and thus difficult to PCR amplify. M, marker, ext., external; int., internal.
