Extended Data Fig. 6: Direction of rectification depends on channel orientation. | Nature Cardiovascular Research

Extended Data Fig. 6: Direction of rectification depends on channel orientation.

From: Kir2.1 dysfunction at the sarcolemma and the sarcoplasmic reticulum causes arrhythmias in a mouse model of Andersen–Tawil syndrome type 1

Extended Data Fig. 6

a, Top, Schematic diagram of expected orientation of SR Kir channel with respect to SR lumen and cytosol in a nuclear membrane experiment. Middle, Representative recordings of SR Kir2.1 currents in control and in the presence of spermine. Bottom, Current voltage relationship and normalized SR Kir2.1 current (same data as in Fig. 6c-e). Each value is represented as mean ± SEM. Statistical analyses were conducted using Two-way ANOVA test. **, p < 0.01; ***, p < 0.001; ****, p < 0.0001. b, Top, Schematic diagram of expected orientation of sarcolemmal Kir channel as reported by Nichols and Lopatin (1996)5. Middle, Representative recordings of sarcolemmal Kir2.1 currents in control and in the presence of spermine. Bottom, Current voltage relationship and normalized sarcolemmal Kir2.1 current. Note that differences between data in a and b are likely related to differences in channel orientation within each lipid bilayer. Figure reproduced with permission of the journal.

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