Extended Data Fig. 8: Chloroquine effects on intracellular Ca2+ dynamics in skeletal myocytes.
a, Representative fluorescence profiles (top) of caffeine-induce calcium release in control conditions and in the presence of Ba2+ 0.5 mM or chloroquine 10 µM in isolated skeletal muscle cells. Graphs (bottom) show the parameters obtained after Boltzman fit (Amplitude: P = 1.000, Control vs Ba2+; P = 0.9771, Control vs CQ; and, P = 0.9771, Ba2+ vs CQ. Tau: P = 0.0445, Control vs Ba2+; P < 0.0001, Control vs CQ; and, P = 0.0011, Ba2+ vs CQ. Baseline: P = 0.0308, Control vs Ba2+; P = 0.0004, Control vs CQ; and, P = 0.1429, Ba2+ vs CQ). Different colors in the same group identify cells coming from one animal. Statistical analysis was conducted using two-level hierarchical one-way ANOVA analysis followed by a Bonferroni’s posttest. Each value is represented as the mean ± SEM. *, p < 0.05; ***, p < 0.001. b, Representative recordings of HEK cells transfected with Kir2.1 channels before and after perfusion with chloroquine 10 µM (P = 0.0282). Graphs show the percentage of block at −120mV. Each value is represented as mean ± SEM. Statistical analyses were conducted using two-tailed paired t-test. *, p < 0.05.
