Extended Data Fig. 4: Mapping of snRNA-seq derived CM subsets to space. | Nature Cardiovascular Research

Extended Data Fig. 4: Mapping of snRNA-seq derived CM subsets to space.

From: Single-cell and spatial transcriptomics of the infarcted heart define the dynamic onset of the border zone in response to mechanical destabilization

Extended Data Fig. 4

(a) Strategy for mapping labels (IZ, RZ, BZ1 and BZ2) from CM nuclei to spatial clusters (see methods). (b) UMAP plot of integrated dataset composed of 34,116 pixels in 16 samples (summarized in Extended Data Fig. 1b, see Extended Data fig. 8). (c) DEGs based on high-resolution clustering (top) and post-classification regions (bottom). (d) Histogram of counts from representative samples (green, sham; red, 72 hrs post-MI). (e) Histogram of BZ1 scores. (f) Dot plot of BZ1 and BZ2 scores. (g,h) Feature plots (g) and violin plots (h) of CM, BZ1 and BZ2 scores. (i) Results of ROC analysis (AUC, area under the curve) and stepwise label-mapping (reference cluster indicated above). (j) Classification results shown in UMAP space. (k) AUC as a function of gene-set length.

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