Extended Data Fig. 3: Assessment of mNPPFC/ConPFC biodistribution, liver serum markers and histology of liver and spleen.
From: Non-invasive mapping of systemic neutrophil dynamics upon cardiovascular injury
a, Biodistribution of intravenously injected mNPPFCs was determined via 19F MRI measurements at distinct time points after injection (1, 3, 6 and 24 h after injection). Shown are quantification of fluorine signal intensities in the blood, liver and spleen indicating a fast wash-out from the blood accompanied by a strong influx into the liver while signal intensities in the spleen were constant over the entire observation period; a.u. = arbitrary units. b, Serum levels of GLDH (glutamate dehydrogenase), AST (aspartate aminotransferase), ALP (alkaline phosphatase), ALT (alanine aminotransferase) and bilirubin after intravenous application of NaCl as control, ConPFCs or mNPPFCs. c + d, 4 µm sections of liver (C) and spleen (D) stained with hematoxylin and eosin. Organs were excised and processed for histology 24 h post injection of NaCl (upper row), ConPFCs (middle), mNPPFCs (lower). Representative liver/spleen images of organs from three different animals are shown. Scale bars indicate 100 µm. Data are mean values ± SD of n = 6 (a), n = 4-6 (b) and n = 6 (c + d) independent experiments.
