Extended Data Fig. 5: ^mNPPFC incorporation into the bone marrow and cardiac function after MI.

a, Impact of neutrophil depletion on ^mNPPFC incorporation into the bone marrow: For in vivo labeling of bone marrow neutrophils, ^mNPPFCs were injected intravenously and 24 h later the ¹⁹F signal within the femur and tibia was determined (gray). Depletion of neutrophils by Ly6G Ab injection 48 and 24 h prior ^mNPPFC injection lead to significantly decreased ¹⁹F bone marrow signals (red). b + c, Locoregional analysis of the femur ¹⁹F signal before and after MI: b, Left: Schematic overview of the femur with the compartments inner/outer epiphysis and diaphysis. The marrow mainly belongs to the diaphysis whereas the growth of the bones takes place at the inner and outer epiphysis. At the right, an exemplarily compartmentalization of a murine ¹H MR scan of the femur with the inner and outer epiphysis as well as the diaphysis is shown. c, Quantification of the ¹⁹F signal in the different femur compartments revealed highest ^mNPPFC uptake in the diaphysis before MI and a significant drop in this compartment after MI. The inner and outer epiphysis are characterized by lower fluorine intensities prior MI and smaller, non-significant decreases upon MI. d + e, Retention of bone marrow neutrophils by NAbs after MI: d, Antibodies against CXCL1, CXCL2, G-CSF and GM-CSF were injected intraperitoneally (i. p.) 1 h before and 4 h post induction of MI to inhibit the MI-stimulated egress of neutrophils from the bone marrow and their infiltration into the heart. Left: Combined ¹H/¹⁹F MRI of the bone marrow (BM) before (left) and after MI (right). e, For quantification, the BM ¹⁹F signal post MI was normalized to the ¹⁹F signals detected before induction of MI. f, Ischemic area and functional cardiac parameters 1 day post MI: To exclude any effects on PFC distribution caused by differences in myocardial tissue impairment after MI between the individual experimental groups, we determined ischemic area, stroke volume and ejection fraction from ¹H MRI LGE cine measurements for each condition (LV = left ventricle). However, no significant differences between ^ConPFCs-, ^mNPPFCs- or ^mNPPFCs + NAbs-treated groups were found. Data are means ± SD of n = 4-5 (a), n = 7 – 11 (c), n = 5 – 8 (e) and n = 5 – 8 (f) independent experiments; * = p < 0.05, verified by two-sided students t-test (a, e) or one-way ANOVA (c).

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