Extended Data Fig. 7: Cross-species analysis of hNP binding and CD177 amino acid sequence.
From: Non-invasive mapping of systemic neutrophil dynamics upon cardiovascular injury
a, hNP does not bind to neutrophils from pigs, rats or mice: Determination of cross-species reactivity of hNP for pig, rat and mouse immune cells (neutrophils, lymphocytes, monocytes) analyzed by flow cytometry. The histogram overlays show binding studies with human immune cells (1st column) and leukocytes isolated from the blood of pigs (2nd column), rats (3rd column) or mice (4th column); gray = untreated cells, red = hNP treated cells. b, Cross-species analysis of CD177 amino acid sequence: Alignment of the protein sequence of CD177 from humans, mice, rats and pigs. Dark blue shows highly conserved sequence areas between all four species. Light blue indicates less conserved amino acids and white represents non-conserved areas.
