Extended Data Fig. 9: Impact of mNPPFCs on murine neutrophils.
From: Non-invasive mapping of systemic neutrophil dynamics upon cardiovascular injury
a, Differentially expressed genes (DEGs) identified by bulk RNA sequencing of murine blood neutrophils after intravenous injection of mNPPFCs, ConPFCs or NaCl (Ctrl) as control. Volcano plots of DEGs for murine neutrophils of animals treated with saline compared to mNPPFCs (left), or ConPFCs compared to mNPPFC (right). Genes marked in red are significantly upregulated with a log2 (fold change) greater than 1.5. BF = Bonferroni corrected p-values of the false discovery rate. In total 25655 RNA transcripts were analyzed. b, Cell surface expression of CD11b, CD62L, CD63 on blood derived murine neutrophils after intravenous application of NaCl (Ctrl) or mNPPFCs. As a positive control, matrigel doped with 50 µg LPS was subcutaneously implanted into the neck of mice and after 24 h, neutrophils were isolated from the blood and analyzed by flow cytometry. c, Impact of mNPPFC incubation on neutrophil migration, phagocytosis and release of reactive oxygen species (ROS). Migration was determined in vivo by assessment of the infiltration of neutrophils into a matrigel/LPS plug; phagocytosis was determined by intravenous injection of FITC-labelled E.coli into mice and the subsequent removal and flow cytometric analysis of blood neutrophils; extracellular ROS were measured within the cell supernatant of blood neutrophils by oxidation of DHE (dihydroethidium) followed by UPLC analysis. Data are shown as means ± SD of n = 6 (a), n = 3-9 (b) and n = 5-7 (c) independent experiments. * = p < 0.05 verified by one-way ANOVA.
