Fig. 2: Correlation of the 19F signal with ischemic area, its temporal development and ex vivo validation.
From: Non-invasive mapping of systemic neutrophil dynamics upon cardiovascular injury
a, Linear regression between ischemic area (LGE) and fluorine signal within the infarcted myocardium. b, Time course of neutrophil infiltration into the injured heart. The fluorine signal was determined 1, 3, 6, 24, 48 and 72 h after induction of MI (examples are given at the top). c, To further corroborate the location of the 19F signal within the infarcted myocardium, hearts were excised, fixed with paraformaldehyde and analyzed by ex vivo high-resolution 1H/19F 3D MRI. In long-axis 1H MR images, the infarcted area can be unequivocally identified as a dark structure within the bright intact myocardium. Importantly, the corresponding 19F signal is restricted to the infarcted area, indicating infiltration of mNPPFC-labeled neutrophils only into the injured myocardium. Left, 3D volume rendering of the heart with superimposed 19F signal. LV, left ventricle; RV, right ventricle. Data are mean ± s.d. of n = 6 (a) and n = 5–6 (b) independent experiments.
