Extended Data Fig. 1: Synthesis scheme for PFC-based nanotracers to target neutrophils.
From: Non-invasive mapping of systemic neutrophil dynamics upon cardiovascular injury
a, Basic structure of the used peptides for targeting neutrophils. The N-terminal carboxyfluorescein is incorporated for fluorescence-based analysis. For coupling the peptides to preformed MalPFCs, a cysteine with a free sulfhydryl group is located at the C-terminus, next to the GGG spacer. The red dashed circle indicates the neutrophil binding sequence. b, For generating NPPFCs, preformed MalPFCs were incubated with the peptides over night to form NP/ConPFCs. MalPFCs were equipped with a separate label (rhodamine) to control for a potential dissociation of binding peptide and PFC. To impair the passive uptake by monocytes/macrophages poly-ethylene-glycol (PEG) coupled lipids (DSPE-PEG2000 = 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(poly-ethylene glycol)-2000]) were inserted in a 5 mol% ratio into the phospholipid shell of PFCs.
