Extended Data Fig. 4: Mitochondrial function regulators are downregulated at DCM onset, and mitochondrial DNA is decreased at end-stage DCM in Kdm8 mutant hearts.
From: KDM8 epigenetically controls cardiac metabolism to prevent initiation of dilated cardiomyopathy
a, Gene ontology terms (cellular component) enriched amongst genes upregulated (yellow) and downregulated (purple) in ventricles of 2-month-old Kdm8 mutant hearts. Data was analyzed using DAVID v6.8 with Benjamini correction. b, qPCR of transcripts of components of electron transport (ETC) complexes and the tricarboxylic acids (TCA) cycle on ventricles of 2-month-old control (Kdm8fl/fl) and mutant (Kdm8fl/fl;Myh6-Cre) hearts, analyzed by two-tailed Student’s t-test with Hold-Sidak multiple comparison correction. n = 5 mice per group. Error bars denote the mean + /- s.e.m. c, qPCR on cardiomyocytes (CMs) and noncardiomyocytes (non CMs) isolated from control and mutant mice at 2 months of age. Error bars denote the mean + /- s.e.m. Data was analyzed by two-way ANOVA. n for Nampt, Ndufa3, and Cox6a2 = 5 control and 6 mutant hearts. n for Ogdh1 = 4 control and 5 mutant hearts. d, qPCR of mitochondrial DNA (mtDNA) normalized to the β-globin gene as representative of nuclear DNA, on the left (LV) and right ventricle (RV) of Kdm8fl/fl control and Kdm8fl/fl;Myh6-Cre mutant hearts, analyzed by two-tailed Student’s t-test. Error bars denote the mean + /- s.e.m. n = 3 to 11 mice per group. qPCR experiments were repeated twice with similar results.
