Extended Data Fig. 5: H3K36me2 is enriched at gene bodies of upregulated genes, and promoters of downregulated metabolic genes in Kdm8 mutant hearts.
From: KDM8 epigenetically controls cardiac metabolism to prevent initiation of dilated cardiomyopathy
a. Proportion of genes with increased or decreased H3K36me2 that were downregulated or upregulated in 2-month-old Kdm8fl/fl;Myh6-Cre mutant hearts. b. Gene ontologies (GO) (biological process) enriched amongst genes whose promoters were enriched for H3K36me2 in 2-month-old Kdm8 mutant hearts. Data was analyzed using DAVID v6.8 with Benjamini correction. c. H3K36me2 CUT&Tag and RNAseq tracks of Acta1 and d. Ogdhl loci in 2-month-old Kdm8fl/fl control and Kdm8fl/fl;Myh6-Cre mutant hearts. The blue line indicates the promoter region upstream the transcription start site of Nampt. Units for CUT&Tag are reads per kilobase per million mapped reads (RPKM) and reads per million mapped reads (RPM) for RNAseq. e. GO terms (biological process) enriched amongst genes downregulated in 2-month-old Kdm8 mutant hearts. Data was analyzed using DAVID v6.8 with Benjamini correction.
