Fig. 1: ALPK3 is a myogenic kinase localized to the M-band of the sarcomere.
a, Uniform manifold approximation and projection (UMAP) plot generated from the snRNA-seq of three non-failing adult human hearts. b, Expression pattern of ALPK3 in cell types of the non-failing adult human heart (colour key shows normalized log(expression) values). c, Schematic of targeting strategy of the ALPK3–tdTomato hPSC cell line and flow cytometry of directed cardiac differentiation for α-actinin and ALPK3–tdTomato. FSC, forward scatter. d, Representative immunofluorescence microscopy image of nanopatterned ALPK3–tdTomato hPSC-derived CMs stained for ALPK3–tdTomato (green), OBSCN (gray) and α-actinin (magenta); scale bar, 5 μm. e, Schematic of targeting strategy of the ALPK3–SBP3×FLAG hPSC cell line and representative immunofluorescence micrograph of WT and ALPK3–SBP3×FLAG hPSC CMs stained for α-actinin (magenta), FLAG (green) and DAPI (blue); scale bar, 10 μm. f, Schematic of targeting strategy of ALPK3–SBP3×FLAG hPSC cell line and western blot of ALPK3 in myofilament and cytosolic fractions of CMs.
