Extended Data Fig. 6: Body weight, food intake, physical activity and respiration in male and female control and ERαHKO mice.
a, Body weight in female and male control (FC) and knockout (KO) mice tracked over time. Data shown as mean±SEM. Male FC n=5, Male KO n=5, Female FC n=8, Female KO n=7. Curves were compared using two-way ANOVA. NS=not significant. b-g, Mice were placed in a metabolic cage on a chow diet for 72h at ~53 weeks of age. Measurements were made in the final 24h. b, Food intake. c, Physical activity assessed via beam breaks over a 12h day/night cycle. d, Averaged oxygen consumption per minute over a 12h day/night cycle. e, Averaged carbon dioxide production per minute over a 12h day/night cycle. f, Respiratory exchange ratio (RER). g, Energy expenditure (EE) relative to grams of lean mass (gLM). For b-g, lines indicate mean±SEM. Male FC n=5, Male KO n=5, Female FC n=8, Female KO n=7; all n represent data from independent animals. In panels b-g, data analyzed with two-sided, unpaired t-test within the same sex. No significant differences between groups.
