Extended Data Fig. 9: Cardiac derived EVs from female ERαHKO mice increased glycolytic capacity in C2C12 myotubes.
The extra cellular acidification rate (ECAR) and oxygen consumption rate (OCR) were measured in differentiated C2C12 myotubes. a, Profiles of glycolytic stress test (ECAR) and Mito Stress Test (OCR) data in differentiated C2C12 myotubes treated for 16 hours with EV’s isolated from ex vivo female mouse cardiac tissues, indicating sequential injections into media of Glucose, Oligomycin, 2-D-Glucose (ECAR) and Oligomycin, FCCP and Rotenone/Antimycin A (OCR). Glycolytic stress test and Mito stress test metabolic parameters as calculated from experimental profiles for C2C12 myotubes treated with b, female derived cardiac EVs and c, male derived cardiac EVs. Values are means ±SEM, 5-6 replicates/EV treatment, P<0.05 considered significant via two-sided, unpaired t-test. FCCP - carbonyl cyanite-4 (trifluoromethoxy) phenylhydrazone. For panel a and b, ECAR: Female FC/KO EVs n=6/5, OCR: Female FC/KO EVs n=5/6. For panel c, ECAR and OCR: Male FC/KO EVs n=5/6. Due to limited material, EVs were pooled from each group of animals (6 independent animals in female FC, female ERαHKO, male FC and male ERαHKO) and n represents individual well replicates for each group as indicated.
