Extended Data Fig. 8: Sample images from selected siRNA knockdowns.
From: Functional analysis of a common BAG3 allele associated with protection from heart failure
(a) HSPB8 knockdown was the only knockdown to significantly reduce BAG3 levels. FLNA, DDB1 and STK38 are BAG3C151R differential interactors whose knockdown resulted in sarcomere scores similar to BAG3 knockdown. ACTN2 and TNNT2 are well known sarcomere components that display low sarcomere scores similar to BAG3 knockdown, possibly due to reduced sarcomeric density and increased disarray and their previously described role as BAG3 client proteins. HSPB7 and DNAJB6 knockdowns displayed high sarcomere scores (similar to Scramble control). (b) Additional micrographs from selected conditions, with stains in separate panels. HSPB8 highlighting reduction of BAG3 levels, and STUB1/CHIP resulting in an increase of BAG3 elongated aggregates. For all images, scale bar = 100 µM. Magenta/Red: BAG3; Green: MYBPC3; Cyan: DAPI. These sample micrographs were selected from a set of randomly acquired images, 9 images per well across 3 separate wells.
