Extended Data Fig. 10: BAG3 variants do not change BAG3 protein levels in iPS-CMs.
From: Functional analysis of a common BAG3 allele associated with protection from heart failure
Capillary immunoassay (SimpleWestern) quantification of BAG3 protein levels in iPS-CM differentiated from iPSCs heterozygous or homozygous for the indicated BAG3 alleles, untreated or treated with Bortezomib (100 nM, 48 h incubation). N = 3 biological replicates from separate differentiation batches. ***: p-value < 0.01; ****: p-value < 0.0001. Two-way ANOVA with Sidak multiple comparison test. Data is presented as mean values +/− SEM.
