Extended Data Fig. 4: Generation of a cell line with inducible expression of the BAG3WT protein.
From: Functional analysis of a common BAG3 allele associated with protection from heart failure
(a) Diagram of the editing strategy. On a BAG3−/− cell background, a doxycycline-activated BAG3-3xFLAG expression cassette was inserted in the PPP1R12C (AAVS1) safe-harbor locus. (b) Western blot of the BAG3 expression on BAG3−/−:TetOn-BAG3WT-3xFLAG iPSCs with and without Doxycycline addition. This immunoblotting reaction was performed twice with similar results.
