Extended Data Fig. 10: Cryo-EM analysis of aspirin-bound ABCC4.
From: Structural insights into human ABCC4-mediated transport of platelet agonist and antagonist
a Representative micrograph and 2D class averages. Bar: 50 nm. The micrograph is a representative of 8,712 cryo-EM images. b The flowchart for cryo-EM data processing. c The angular distribution plot of the final 3-D reconstruction of aspirin-bound ABCC4. d FSC curves for aspirin-bound ABCC4. e The local resolution map of aspirin-bound ABCC4. The color code for resolutions, shown with the unit Å, is calculated using CryoSPARC. f Cryo-EM maps for representative segments of aspirin-bound ABCC4. Contour levels for TM1-12, the lasso motif, the aspirin molecule and coordinating TMs are set at 5σ.
