Extended Data Fig. 1: Megakaryocytes and platelets have unique lipidomic profiles.
Murine bone marrow cell populations were isolated by fluorescence-activated cell sorting and platelets by sequential centrifugation. Lipids were extracted and analyzed using 20-min gradient HPLC and mass spectrometry (see methods for details). (a) Percentage of different lipid classes of indicated murine bone marrow cell populations and autologous platelets in lipidomic analyses. Volcano plots from the different lipid classes between (b) bone marrow extracellular fluid (BMEF) and MEPs. n = 4 and n = 8, respectively, (c) BMEF and immature (CD41+) MKs, n = 4 (d) BMEF and mature (CD41/42+) MKs, n = 4, and (e) platelets and plasma n = 4. Total percentage of all acyl/alkyl composition of (f) PC (g) PI, (h) PE, (i) PS. n = 8 for MEP and 4 for all other cell types, biological replicates. All data are presented as mean +/− SD. MK: megakaryocyte; MEP: MK-erythroid progenitor; PA: phosphatidic acid; PC: phosphatidylcholine; PE: phosphatidylethanolamine; PI: phosphatidylinositol; PS: phosphatidylserine; PG: phosphatidylglycerol.
