Extended Data Fig. 6: JOSD2 regulates calcium (Ca2+) handling in cardiomyocytes. | Nature Cardiovascular Research

Extended Data Fig. 6: JOSD2 regulates calcium (Ca2+) handling in cardiomyocytes.

From: Deubiquitinase JOSD2 improves calcium handling and attenuates cardiac hypertrophy and dysfunction by stabilizing SERCA2a in cardiomyocytes

Extended Data Fig. 6: JOSD2 regulates calcium (Ca2+) handling in cardiomyocytes.The alternative text for this image may have been generated using AI.

(a) Specimen records of caffeine (10 mM)- induced determination of sarcoplasmic reticulum (SR) Ca2+ content in AVCMs isolated from WT or Josd2−/− mice under TAC treatment. (b) The expression efficiency of JOSD2 in NRPCs transfecting with plasmids (left) or siRNA (right) was verified by immunoblot. (n = 3, EV= empty vector, NC=negative control) (c) Representative confocal images and recordings of cytosolic Ca2+ as determined by the Fluo-4 AM fluorescence signal in NRPCs during spontaneous beats. NRPCs were transfected with plasmids (Upper) or siRNA (Below) before Ang II (1 μM) stimulation for 12 h (n = 6, P values were determined by one-way ANOVA with Bonferroni’s correction and data are presented as mean ± SEM).

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