Extended Data Fig. 7: Cardiac-specific overexpression of JOSD2 or SERCA2a in Ang II-induced mice. | Nature Cardiovascular Research

Extended Data Fig. 7: Cardiac-specific overexpression of JOSD2 or SERCA2a in Ang II-induced mice.

From: Deubiquitinase JOSD2 improves calcium handling and attenuates cardiac hypertrophy and dysfunction by stabilizing SERCA2a in cardiomyocytes

Extended Data Fig. 7

AAV9 over-expressing JOSD2 (JOSD2OE), SERCA2a (SERCA2aOE), or vehicle (EV) were injected in WT or Josd2−/− mice through tail vein (a total dose of 2E + 11 vg, injected twice, 4 weeks before osmotic pumps implantation). These mice were then implanted with Ang II osmotic pumps. (a, b) Representative immunoblot of Flag-JOSD2 and SERCA2a in heart tissues. (c) Lysates of heart tissue from JOSD2OE mice were subjected to Co-IP with anti-SERCA2a, which was followed by immunoblot with an anti-Ub. (d, e) Mice body weight (n = 6 for each group) and systolic blood pressure (SBP, n = 3-4) during study. (For d and e, data are presented as mean ± SEM).

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