Extended Data Fig. 1: Identification of JOSD2 as a regulator in cardiac hypertrophy.
(a) The expression profile of Machado-joseph deubiquitinases (MJDs) family in heart tissues of Ang II mice. Construction of transcriptome sequencing library and transcriptome sequencing was completed by LC-Bio Technology Co., Ltd. (Hangzhou, China). mRNA sequences number was normalized by fragments per kilobase million (FPKM) (n = 3). (b) The silence efficiency of Josd1, Josd2 and Ataxin-3 in NRPCs was verified by RT-qPCR (NC=negative control; n = 3). For a and b, P values were determined by two-tailed unpaired t-test and data are presented as mean ± SEM. (c) Representative immunoblot for JOSD2 in Ang II-induced cardiac fibroblasts (n = 3). (d) Representative immunofluorescence staining for JOSD2 (green) and α-actin (red), vimentin (red) or CD68 (red) in heart sections from Ang II infusion or TAC operation mice. Merged images (yellow) showing colocalization (n = 3).
