Extended Data Fig. 9: Analysis of the megakaryocytes upon inhibition of phospholipid biosynthesis. Related to Fig. 5.
From: Critical shifts in lipid metabolism promote megakaryocyte differentiation and proplatelet formation
a, Lipidome analysis showing the relative quantities (mean ± SD, n = 4) per lipid class of control and inhibitor treated MKs on day 7. Non-treated day 0 MKs were used as baseline control and lipid quantities were set to 1 and the standard deviation is depicted as dotted lines. Changes for all lipid classes were calculated as ratios relative to control day 0. A two-sided t-test was used for statistical analysis. All days were tested against day 7 control (*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001). b, Arithmetic means ± SD (n = 5-7) of MKs of Triacsin C, FSG67 or vehicle treated mice per visual field of in vivo imaging in the BM vasculature of the frontoparietal skull of mice. c, Arithmetic mean ± SD (n = 5-7) of MKs and MK area of Triacsin C, FSG67 or vehicle treated mice per visual field in immunostained BM sections.
