Extended Data Fig. 10: Lipid-dependent kinases in MKs involved in megakaryopoiesis and proplatelet formation. Related to Fig. 6.
From: Critical shifts in lipid metabolism promote megakaryocyte differentiation and proplatelet formation
a, Network showing significantly regulated kinases (blue) and lipid-binding proteins (yellow). Edges are correlations with a minimum interaction confidence score 0.5. Nodes are proteins. b, Domain composition of depicted kinases. Membrane-targeting modules are the C1 (orange), C2 (yellow), pleckstrin homology (PH) or PH-like (purple), and FERM (light blue) domains. Additional interaction domains like Src-homology (SH) domains are shown in dark blue. Kinase domains are shown in gray. Note, the schematic representation is not reproduced in the correct domain proportions and does not reflect the actual protein size. c, Regulation of kinases with a lipid binding domain (mean ± SD, n = 3). A two-sided t-test was used for statistical analysis. Benjamini Hochberg correction was applied to p-values using an FDR cut-off of 0.05 (*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001). d, Arithmetic means of MKs and MK area of csnk2α1lox/lox and csnk2α1Pf4∆/Pf4∆ mice per visual field (n = 30) in immunostained BM sections.
