Fig. 4: Quantitative lipid inventory analysis of MKs reveals shifts in FA and double bond distribution during the course of MK maturation.
From: Critical shifts in lipid metabolism promote megakaryocyte differentiation and proplatelet formation
a, Relative lipid class distribution at different time points (dark, day 1; light, day 3; medium, day 7) of MK maturation. b, Absolute concentrations of selected lipid classes. D1, day 1; D3, day 3; D7, day 7. c, Selected lipid species, each representing the corresponding class displayed in b. d, Amount and distribution of FA chain length (number of carbon atoms) (top) and double bonds (bottom) of GPs on days 1, 3 and 7 of MK maturation. e, Bar graphs of FA absolute abundances derived from different GP species. f, Absolute quantification of the saturated, monounsaturated and polyunsaturated FA content of GPs during megakaryopoiesis (on days 1, 3 and 7) and in platelets (PLT). All data show the mean of three biological replicates. One biological replicate was comprised of five individual animals. Error bars represent standard deviations. In f, error bars represent summed standard deviations of the total FA content each day. A two-sided t-test was used for statistical analysis (*P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001).
