Extended Data Fig. 1: Lipidomic profiling of platelets from AngII-infused mice.
a-c. Principal component analysis of lipidomic data on phospholipids (a) and quantitative measurement of Ceramides, SM, PC, PE, Lyso-PC and Lyso-PE by mass spectrometry (b-c) in circulating platelets from 16-week-old male ApoE-/- mice infused with saline, AngII (1000 ng/kg/min), AngII plus losartan (in drinking water, 60 mg/kg/day) or AngII plus aspirin (in drinking water, 3 mg/kg/day) for 7 days. Each point represents an individual mouse sample (a). n = 12 mice per group, P values were calculated by Krustal-Wallis test followed by Dunn’s test (b-c). d. Platelets were isolated from 16-week-old male ApoE-/- mice infused with saline or AngII (1000 ng/kg/min) for 7 days, followed by further incubation for 30 min. Mass spectrometric measurement of ceramides in conditional media of isolated mouse platelets. n = 3 biologically independent experiments, P values were calculated by the two-sided Mann-Whitney test; ND, not detected. e. Mass spectrometric measurement of plasma ceramides from 16-week-old male ApoE-/- mice infused with saline or AngII (1000 ng/kg/min) for 7 days. n = 12 mice per group, P values were calculated by two-sided Mann-Whitney test. f. Quantitative measurement of ceramide by mass spectrometry in plasma from 16-week-old male ApoE-/- mice infused with saline or AngII (1000 ng/kg/min) for 28 days. n = 7 mice per group, P values were calculated by the two-sided Mann-Whitney test. Data are presented as mean ± SEM.
