Extended Data Fig. 5: Silencing MMP16 expression has no effect on osteogenic phenotype switching in human coronary artery vascular smooth muscle cells. | Nature Cardiovascular Research

Extended Data Fig. 5: Silencing MMP16 expression has no effect on osteogenic phenotype switching in human coronary artery vascular smooth muscle cells.

From: Whole-genome sequencing uncovers two loci for coronary artery calcification and identifies ARSE as a regulator of vascular calcification

Extended Data Fig. 5: Silencing MMP16 expression has no effect on osteogenic phenotype switching in human coronary artery vascular smooth muscle cells.

a) Treatment of human coronary artery vascular smooth muscle cells (n = 6 biologically independent samples in each group) with osteogenic media decreased MMP16 mRNA expression by ~74% (left panel). Treatment of cells grown in osteogenic media with siMMP16 (resulting in >90% knockdown of MMP16 mRNA) had no effect on RUNX2 (middle panel) or CNN1 (right panel) mRNA levels. Statistical comparisons were made using a two-tailed one-way ANOVA with Sidak’s post-hoc comparison testing. The mean ± SEM is depicted in plots. b) Treatment of human coronary artery vascular smooth muscle cells grown in osteogenic media with siMMP16 had no effect on calcification, as evidenced by Alizarin Red S staining.

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