Fig. 1: Myocardial IR induces a reduction in MerTK+ cardiac macrophage proportions. | Nature Cardiovascular Research

Fig. 1: Myocardial IR induces a reduction in MerTK+ cardiac macrophage proportions.

From: ATF3 coordinates the survival and proliferation of cardiac macrophages and protects against ischemia–reperfusion injury

Fig. 1: Myocardial IR induces a reduction in MerTK+ cardiac macrophage proportions.

a, UMAP of seven macrophage (Mφ)/monocyte (mon) clusters identified via scRNA-seq analysis. b, UMAP visualization of MerTK+CCR2− and MerTK−CCR2+ macrophages. c, Split UMAP of macrophage/monocyte clusters (left) and percentage of seven clusters (right) in the heart between sham operation and IR. d, Representative flow cytometry plots (left) and quantification (right) of MerTK+CCR2− macrophages and MerTK−CCR2+ macrophages under sham operation and IR (n = 6 mice per group). e,f, Quantification (e) and colocalization (f) of IF signals of F4/80 and LYVE1, TREM2 and CD74 in cardiac infarct regions of the sham and IR (n = 6 mice per group). Scale bars, 20 μm. Statistical significance was evaluated using two-tailed, unpaired Student’s t-test (d: MerTK macrophage (Mφ), MHCII Mφ, Lyve1 Mφ, Trem2 Mφ; and f) or unpaired Mann–Whitney U-test (d: CCR2 Mφ). All data are presented as mean ± s.e.m. HPF, high-power field.

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