Extended Data Fig. 8: Cardiac repair is attenuated upon the loss of myeloid-specific ATF3.
a. Representative transverse cardiac magnetic resonance imaging (MRI). The epicardial border (green), endocardial border (red), and infarct region (yellow) are delineated on a T1 map (up). Representative images of Evans blue and 2,3,5−triphenyltetrazolium chloride staining (down) in ATF3-CKO and WT hearts 1 day post-IR (bottom). Scale bar, 1 mm. b. Late gadolinium enhancement was used to determine the infarct size (n = 6 mice per group). c. Ejection fraction (EF) as determined via MRI 1 day post-IR (n = 6 mice per group). d. Infarct size was calculated as a percentage of the myocardial area at risk (sham, n = 6 mice per group, IR-1d, n = 10 mice per group). e. Representative images and quantification of TUNEL+α-actinin+ cardiomyocytes in the hearts of ATF3-CKO and WT mice 1 day post-IR (n = 6 mice per group). Scale bar, 50 μm. f. Flow cytometry analysis and quantification of CD45-PDGFRα-CD31+ EC populations in the hearts of ATF3-CKO and WT mice (n = 6 mice per group). g. Representative images and quantification of CD31+ area per HPF in CD31+ cells in the cardiac border area of ATF3-CKO and WT mice (n = 6 per group). Scale bar, 20 μm. h, i. Representative images (h) and quantification (i) of microfil vascular casting and microCT in ATF3-CKO and WT hearts 7 days after IR (n = 6 mice per group). Scale bar, 1 mm. j. Representative echocardiographic images and ejection factor of ATF3-CKO and WT mice 30 days after IR (n = 6 mice per group). k. Representative images and quantification of fibrosis of ATF3-CKO and WT hearts 30 days after IR (n = 6 mice per group). Scale bar, 1 mm. Statistical significance was evaluated using two-tailed two-way ANOVA analysis followed by Tukey’s multiple comparisons test (b-g, and i-k). All data are presented as mean ± SEM.
