Extended Data Fig. 7: Increased SR Ca2+ content in TS1 ventricular myocytes.
a-b, SR Ca2+ content measured by two simultaneous methods: caffeine-induced Ca2+ release and its concomitant Transient Inward current, a reliable indicator of the efflux of the released Ca2+ by the Na+/Ca2+ exchanger. Both methods demonstrate that the SR Ca2+ content is higher in TS1 (red) as compared to WT (blue) cardiomyocytes. a, SR Ca2+ content measurements (shown 1 Hz). b, Pooled data of caffeine peak in WT (blue, n = 15/9/7 cells from N = 3/3/3 animals, at 0.5, 1, and 2 Hz, respectively) and TS1 cardiomyocytes (red, n = 24/14/9 cells from N = 4/4/3 animals at 0.5, 1, and 2 Hz, respectively). c, Pooled data of Transient Inward current time-integral in WT (blue, n = 12/9/7 cells from N = 3/3/3 animals, at 0.5, 1, and 2 Hz, respectively) and TS1 cardiomyocytes (red, n = 21/13/9 cells from N = 4/4/3 animals at 0.5, 1, and 2 Hz, respectively). Each value is represented as mean ± s.e.m. Statistical analyses were conducted using two-tailed nested t-test. N.S. not significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
