Extended Data Fig. 8: NCX current comparison in cardiomyocytes from wild-type and TS1 pigs.
a, NCX currents were recorded in isolated WT (top-left; blue) and TS1 (center-left; red) ventricular cardiomyocytes, in response to the voltage protocol shown below (bottom-left). Currents were first recorded in the absence (black traces) and then in the presence (grey traces) of 5 mM Nickel, a NCX inhibitor. Thus, NCX currents were obtained by subtraction (blue trace at the top-left panel and red trace at the center-left panel). b, I-V curve of the pooled data of WT (top-left; blue, n = 21 cells from N = 5 animals) and TS1 (center-left; red, n = 25 cells from N = 5 animals) cardiomyocytes, represented at 10 mV intervals. Each value is represented as mean ± s.e.m. Statistical analyses were conducted using two-tailed nested t-test (p values shown in the figure for each 10 mV interval). N.S. not significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
