Extended Data Fig. 2: Venn diagram visualization, principal component analysis, and unsupervised hierarchical clustering of the phosphoproteome datasets. | Nature Cardiovascular Research

Extended Data Fig. 2: Venn diagram visualization, principal component analysis, and unsupervised hierarchical clustering of the phosphoproteome datasets.

From: Cysteine-rich with EGF-like domains 2 (CRELD2) is an endoplasmic reticulum stress-inducible angiogenic growth factor promoting ischemic heart repair

Extended Data Fig. 2

(a–c) Phosphoproteome analysis of human coronary artery endothelial cells stimulated for 5 or 15 min with CRELD2 (100 ng/mL) or vascular endothelial growth factor (VEGFA, 50 ng/mL); 4 technical replicates per time point and condition. (a) Venn diagrams showing the numbers of significantly up- (red) or downregulated (blue) phosphosites in CRELD2 or VEGFA-stimulated cells. (b) Principal component analysis. (c) Dendrograms depicting hierarchical clusters based on 499 significantly regulated phosphosites (Benjamini-Hochberg false discovery rate-corrected ANOVA, P < 0.05) using Ward’s method and Euclidian distance as a similarity metric.

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