Extended Data Fig. 5: Lack of CRELD2 effects in murine embryonic fibroblasts. | Nature Cardiovascular Research

Extended Data Fig. 5: Lack of CRELD2 effects in murine embryonic fibroblasts.

From: Cysteine-rich with EGF-like domains 2 (CRELD2) is an endoplasmic reticulum stress-inducible angiogenic growth factor promoting ischemic heart repair

Extended Data Fig. 5

(a) EdU incorporation in mouse embryonic fibroblasts (MEFs) cultured for 24 hours in the absence or presence of transforming growth factor beta 1 (TGFβ1, 2 ng/mL) or CRELD2 (100 ng/mL). 3–6 experiments. (b) Recovery 16 hours after scratch injury of MEF monolayers in the absence or presence of TGFβ1 or CRELD2. 4 experiments. (c) Smooth muscle actin alpha 2 (Acta2) and (d) collagen type I alpha1 (Col1a1) mRNA expression (RT-qPCR, normalized to glyceraldehyde-3-phosphate dehydrogenase [Gapdh]) in MEFs cultured for 24 hours in the absence or presence of TGFβ1 or CRELD2. 6 experiments. (a–d) *P < 0.05, **P < 0.01, ***P < 0.001 (1-way ANOVA with Tukey test). Individual data points, mean values, and SEMs are shown. (e) Exemplary immunoblots (out of 3) showing phosphorylated (P)-SMAD2 (S465/S467), P-SMAD3 (S423/S425), and SMAD2 and SMAD3 expression in MEFs stimulated with TGFβ1 in the absence or presence of CRELD2 (added 30 min prior to TGFβ1).

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