Extended Data Fig. 6: Effect of systemic and T cell-specific CCR8 deficiency on atherosclerosis and analysis of CCR1 and CCR5 expression in different T cell subsets.

(a) Experimental scheme of CD4^Cre-Ccr8^flox/floxApoe^−/− (CD4Ccr8^WTApoe^−/−) and CD4^Cre+Ccr8^flox/flox Apoe^−/− (CD4Ccr8^KOApoe^−/−) mice fed a WD for 12 weeks; (b) Representative images and quantification of lesion area measured after HE-staining in the aortic root of CD4Ccr8^WT Apoe^−/− (n = 16) or CD4Ccr8^KO Apoe^−/− (n = 9) mice. Scale bar = 500 µm; (c) Quantification of lesion area measured after Oil-Red-O staining for lipid deposits in the thoraco-abdominal aorta of CD4Ccr8^WT Apoe^−/− (n = 17) or CD4Ccr8^KO Apoe^−/− (n = 8) mice. (d) Atherosclerotic lesion size in the aortic arch, as quantified after H&E staining in CD4Ccr8^WT Apoe^−/− (n = 14) or CD4Ccr8^KO Apoe^−/− (n = 8) mice. (e) Experimental scheme of CD4Ccr8^WT Apoe^−/− or CD4Ccr8^KO Apoe^−/−mice fed a WD for 12 weeks; (f-j) Representative images and quantification of the percentage of lesional Mac2⁺ macrophages (f,g) or SMA⁺ SMCs (h,i) and collagen content (j) in aortic arch sections of CD4Ccr8^WT Apoe^−/− (g,i,j all n = 20) or CD4Ccr8^KO Apoe^−/− (g,i,j all n = 23) mice fed a WD for 12 weeks. Scale bar = 250 µm; (k,l) Ccr1 and Ccr5 mRNA expression in different T cell populations of the para-aortic LNs from Apoe^−/−Ccl17^e/wt and Apoe^−/−Ccl17^e/e mice fed a WD for 6 weeks; (m) Flow cytometric quantification of CD45⁺CD3⁺CD4⁺FoxP3⁺Tbet⁺ cells in aortic, axillary and mesenteric LNs of Apoe^−/−, Apoe^−/−Ccl3^−/− or Apoe^−/−Ccl17^e/e mice (all n = 8). (a-m). Data represent mean ± SEM. Two-sided P values as indicated and analyzed by by Mann-Whitney U test or unpaired Student’s t-test (b-j), multiple Mann-Whitney U test with false discovery rate (FDR) (k,l), one-way ANOVA with Holm-Šídák’s post hoc test (aortic and axillary LNs) or Kruskal-Wallis H with Dunn’s post hoc test (mesenteric LNs) (m).

Source data