Extended Data Fig. 3: Additional analyses of atherosclerosis (related to Fig. 2).
From: TREM2 protects from atherosclerosis by limiting necrotic core formation
a) Atherosclerotic lesion formation in the aorta at 8 weeks of HFD feeding (n = 7 Trem2+/+ BM, n = 9 Trem2−/− BM, all males) and b) atherosclerotic lesion formation in the innominate artery (n = 13 Trem2+/+ BM, n = 14 Trem2−/− BM, all males) and aorta at 12 weeks of HFD (n = 9 Trem2+/+ BM, n = 11 Trem2−/− BM, all males) in Ldlr−/− mice irradiated and reconstituted with Trem2+/+ or Trem2−/− bone marrow. Data in a and b presented as mean +/− SEM together with individual data point distribution, statistical test: two-tailed Mann Whitney test (a, b for en face aorta); unpaired two-tailed t test (b for innominate artery). c) representative pictures of macrophage coverage (Mac2 staining), related to Fig. 2f (8 weeks HFD; n = 7 Trem2+/+ BM, n = 9 Trem2−/− BM, all males), Fig. 2g (12 weeks HFD; n = 14 Trem2+/+ BM, n = 15 Trem2−/− BM, all males), Fig. 2h (16 weeks HFD; n = 6 Trem2+/+ BM, n = 7 Trem2−/− BM, all males), and Fig. 2i (20 weeks HFD; n = 11 Trem2+/+ BM, n = 8 Trem2−/− BM, all males). d) representative pictures of macrophage coverage (Mac2 staining) in in Ldlr−/− mice fed a high fat diet and treated with isotype antibody or 4D9 for 10 weeks (5mg/kg i.p. twice weekly), related to Fig. 2p (n = 14 Ldlr−/− mice treated with isotype control (8 males, 6 females); 17 Ldlr−/−, mice treated with 4D9 (10 males, 7 females)).
