Extended Data Fig. 5: Analysis of atherosclerosis in Ldlr−/− mice treated with low dose TREM2 agonist antibody 4D9.
From: TREM2 protects from atherosclerosis by limiting necrotic core formation
a) Lesion coverage in the en face aorta of Ldlr−/− mice treated with 4D9 as in Fig. 2n-q (5mg/kg twice weekly, 10 weeks high fat diet (HFD)); n = 8 Ldlr−/− mice treated with isotype control (3 males, 5 females); 11 Ldlr−/− mice treated with 4D9 (5 males, 6 females), squares: female mice, circles: male mice. b-e) analysis of atherosclerosis in mice treated with low dose 4D9 (1mg/kg weekly, 9 weeks high fat diet) with b) lesion coverage in the en face aorta (n = 19 Ldlr−/− mice treated with isotype control, n = 17 Ldlr−/− mice treated with 4D9), c) lesion size in the aortic sinus (n = 17 Ldlr−/− mice treated with isotype control, n = 16 Ldlr−/− mice treated with 4D9), d) macrophage coverage in aortic sinus lesions (n = 18 Ldlr−/− mice treated with isotype control, n = 16 Ldlr−/− mice treated with 4D9) and e) relative necrotic core size in aortic sinus atherosclerotic lesions (n = 19 Ldlr−/− mice treated with isotype control, n = 17 Ldlr−/− mice treated with 4D9); data in b-e pooled from two experiments, all male mice. Data presented as mean +/− SEM together with individual data point distribution. Statistical test: two-tailed unpaired t test (all panels). Pictures in a and b created with BioRender.com.
