Extended Data Fig. 6: Single-nucleus RNA-seq of Ldlr−/−Trem2−/− aortas. | Nature Cardiovascular Research

Extended Data Fig. 6: Single-nucleus RNA-seq of Ldlr−/−Trem2−/− aortas.

From: TREM2 protects from atherosclerosis by limiting necrotic core formation

Extended Data Fig. 6: Single-nucleus RNA-seq of Ldlr−/−Trem2−/− aortas.

a) Experimental design; b) UMAP visualization of aortic single-nucleus transcriptomes from Ldlr−/−Trem2+/+ (n = 3 females) and Ldlr−/−Trem2−/− (n = 3 females) mice after 10 weeks of HFD feeding (MPC:mononuclear phagocytes, VSMC: vascular smooth muscle cells) and c) expression of marker genes defining cell lineages; d) UMAP plot of mononuclear phagocytes after subsetting and reclustering (Mac: macrophage; Myelo: myeloid cell; DC: dendritic cell; nd: not determined); e) marker genes of mononuclear phagocyte clusters; f) expression of selected differentially expressed genes in foamy macrophages as determined from pseudo-bulk analysis of experimental replicates from the snRNA-seq data. Samples with >200 foamy macrophages were included for pseudo-bulk analysis. Picture in a created with BioRender.com.

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